WebApr 16, 2024 · In this conversation. Verified account Protected Tweets @; Suggested users This paper by the Henikoff lab introduces CUTAC, a molecular tool that allows users to sequence DNA inside nucleosome depleted regions accessible to transposition by a protein A (pA)-Tn5 fusion protein. CUTAC builds on the Henikoff lab's exciting new CUT&TAG method.
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WebApr 15, 2024 · II (RNAPII). “CUTAC” (Cleavage Under Targeted Accessible Chromatin) is conveniently performed in parallel with ordinary CUT&Tag, producing accessible site maps from low cell numbers with signal-to-noise as good as or better than the best ATAC-seq datasets. Results Streamlined CUT&Tag produces high-quality datasets with low cell … WebHatice S Kaya-Okur 1 2 3 , Derek H Janssens 1 , Jorja G Henikoff 1 , Kami Ahmad 1 , Steven Henikoff 4 5 Affiliations 1 Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA. 2 Howard Hughes Medical Institute, Chevy Chase, MD, USA. 3 Altius Institute for Biomedical Sciences, Seattle, WA, USA. 4 Basic ... proverbs activity
Simultaneous CUT&Tag profiling of the accessible …
WebCUT&Tag-direct with CUTAC. Version 2. Steven Henikoff 1, Jorja Henikoff 1, Hatice S Kaya-Okur 1, Kami Ahmad 1. 1 Fred Hutchinson Cancer Research Center. Steven Henikoff. Workspaces. Aug 12, 2024. Publication. CUT&Tag Data Processing and Analysis Tutorial. Ye Zheng 1, Kami Ahmad 1, Steven Henikoff 1. WebJan 17, 2024 · A triplicate of positive control was prepared following the standard tagmentation protocol including Zymo cleaning to remove Tn5 transposase and the PCR step to amplify tagmented DNA. All purified DNA products were then diluted 1:10 in 10 mM Tris, pH 8.0 and quantitated using Picogreen and a lambda DNA standard. WebJun 24, 2024 · Previously, we described a novel alternative to chromatin immunoprecipitation, CUT&RUN, in which unfixed permeabilized cells are incubated with antibody, followed by binding of a protein A-Micrococcal Nuclease (pA/MNase) fusion protein (Skene and Henikoff, 2024). Here we introduce three enhancem … restart upon crash