T4 ligase hc
WebT4 RNA Ligase 1 Add to Cart: L6050L: 10,000 U: 20,000 U/ml: $443: Single-stranded RNA ligase. Also joins single-stranded DNA molecules. Add to Cart: T4 DNA Ligase (Rapid) Add to Cart: L6030-HC-L: 240,000 U: 600,000 U/ml: $707 “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008). Add to Cart: T3 DNA Ligase Add to Cart ... WebT4 DNA Ligase (Rapid) Description [600,000 Units/ml] 240,000 Units (0.40 ml) Share your knowledge of this product with other customers... Be the first to write a review Browse for more products in the same category as this item: Ultrapure Ligases
T4 ligase hc
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WebB6030 (10X T4 DNA Ligase Buffer) 10X T4 DNA Ligase Buffer (B6030) 500mM Tris-HCI 100 mM MgCl 2 50 mM DTT 10 mM ATP pH 7.6 @ 25°C Unit Definition 1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X T4 DNA Ligase Buffer following a 30 minute incubation at … WebOct 16, 2012 · T4 DNA Ligase. To Request Technical Support. Fill out our Technical Support Form, email us, or call 1-800-632-7799. For Questions Related to NEB Products …
WebThe T4 DNA Ligase requires ATP as a cofactor. Highlights. Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) Fast – sticky … WebT4 DNA Ligase ( NEB #M0202) Is the enzyme of choice for the majority of recombinant DNA applications. It can be used for for cohesive ends (10 mins RT) and blunt ends (2 …
WebT4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single … WebCatalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single ...
WebT4 DNA Ligase (Rapid) Part Number L6030-HC-L Concentration 600,000 U/mL Unit Size 240,000 U Product Information Sheet L6030-HC-L Rev F Product Specification Storage …
WebT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5ʹ phosphate and a 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins … tourismus bachelorarbeitWebT4 DNA ligase catalyzes the formation of phosphodiester bonds between adjacent 3′-hydroxyl and 5′-phosphaste termini in double-stranded DNA. The ligase commercially available is now made by recombinant DNA means. T4 DNA ligase is a single polypeptide of 68 kDa; the enzyme requires Mg 2+ and ATP as cofactors. tourismusbericht 2021Web10X T4 DNA Ligase Buffer (B603) 500mM Tris-HCI 100 mM MgCl 2 50 mM dithiothreitol 10 mM ATP pH 7.6 @ 25°C Molecular Weight 55.3 kDa Unit Definition 1 unit is defined as … tourismusberichthttp://www.enzymatics.com/pdf/L603-HC-L.pdf pottery tiles artWebSep 21, 2016 · So for T4 DNA ligase the catalytic activity at room temperature might be less than when compared to 16 degree celsius. The time also has a role to play as if you don't incubate longer you are... pottery time slotWebSource of Protein: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene. Unit Definition: 1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C. Molecular weight: 55,292 Daltons. Quality Control ... tourismus apfellandWebGlycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50U/µL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate and 3' hydroxyl groups in duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. tourismus altdorf